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Images containing sensory nerve fibers within the subbasal layer between the Bowman layer and the basal epithelium were further analyzed

Blastocyst formation rate: Defined as the proportion of 2PN zygotes (not just of cleaved zygotes) which are at the blastocyst stage at Day 5 (116 2 h post-insemination) (18), We employed the Gardner system to evaluate the quality of blastocysts in terms of their expansion, inner cell mass, and trophoblast development (20)

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